› 6swg

X-ray diffraction
2.51Å resolution

Crystal structure of the TASOR-Periphilin core complex

Released:
DOI: 10.2210/pdb6swg/pdb
PDB entry 6swg coloured by chain, front view.
PDB entry 6swg coloured by chain, side view.
PDB entry 6swg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Periphilin self-association underpins epigenetic silencing by the HUSH complex.
Prigozhin DM, Douse CH, Farleigh LE, Albecka A, Tchasovnikarova IA, Timms RT, Oda SI, Adolf F, Freund SMV, Maslen S, Lehner PJ, Modis Y
Nucleic Acids Res 48 10313-10328 (2020)
PMID: 32976585

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185518 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periphilin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 11.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8NEY8 (Residues: 285-341; Coverage: 12%)
Gene names: HSPC206, HSPC232, PPHLN1
Sequence domains: Transcriptional regulatory protein LGE1
Protein TASOR Chain: C
Molecule details ›
Chain: C
Length: 83 amino acids
Theoretical weight: 9.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UK61 (Residues: 1013-1095; Coverage: 5%)
Gene names: C3orf63, FAM208A, KIAA1105, TASOR

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3221
Unit cell:
a: 93.57Å b: 93.57Å c: 84.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

RNA-mediated heterochromatin formation at repetitive elements in mammals.
Stamidis et al. (2023)
2 more

1 mention without citation

Periphilin self-association underpins epigenetic silencing by the HUSH complex.
Prigozhin et al. (2020)