› 6sxa

Electron Microscopy
3.6Å resolution

XPF-ERCC1 Cryo-EM Structure, Apo-form

Released:
DOI: 10.2210/pdb6sxa/pdb
PDB entry 6sxa coloured by chain, front view.
PDB entry 6sxa coloured by chain, side view.
PDB entry 6sxa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of the XPF-ERCC1 endonuclease reveal how DNA-junction engagement disrupts an auto-inhibited conformation.
Jones M, Beuron F, Borg A, Nans A, Earl CP, Briggs DC, Snijders AP, Bowles M, Morris EP, Linch M, McDonald NQ
Nat Commun 11 1120 (2020)
PMID: 32111838
Related structures: EMD-10337

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139875 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair endonuclease XPF Chain: F
Molecule details ›
Chain: F
Length: 916 amino acids
Theoretical weight: 104.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92889 (Residues: 1-916; Coverage: 100%)
Gene names: ERCC11, ERCC4, XPF
Sequence domains: ERCC4 domain
DNA excision repair protein ERCC-1 Chain: G
Molecule details ›
Chain: G
Length: 297 amino acids
Theoretical weight: 32.6 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07992 (Residues: 1-297; Coverage: 100%)
Gene name: ERCC1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-10337
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Targeting Drug Chemo-Resistance in Cancer Using Natural Products.
Talib et al. (2021)
3 more

4 mentions without citation

Targeting protein-protein interactions in the DNA damage response pathways for cancer chemotherapy.
McPherson et al. (2021)
3 more