› 6sy0

X-ray diffraction
3.1Å resolution

Structure of the Plasmodium falciparum SIP2 DNA-binding AP2 tandem repeat in complex with two SPE2 half-sites

Released:
DOI: 10.2210/pdb6sy0/pdb
PDB entry 6sy0 coloured by chain, front view.
PDB entry 6sy0 coloured by chain, side view.
PDB entry 6sy0 coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Entry authors: Reiter D, Kantsadi A, Vakonakis I

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111601 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
AP2 domain transcription factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 139 amino acids
Theoretical weight: 16.62 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6KSN9 (Residues: 173-311; Coverage: 7%)
Gene name: PF3D7_0604100
DNA (5'-D(*GP*GP*TP*GP*CP*AP*CP*CP*TP*AP*GP*GP*TP*GP*CP*AP*CP*C)-3') Chains: E, F, G, H, K, L
Molecule details ›
Chains: E, F, G, H, K, L
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CAF 3 x CAF

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P2
Unit cell:
a: 80.35Å b: 43.72Å c: 153.71Å
α: 90° β: 92.17° γ: 90°
R-values:
R R work R free
0.276 0.274 0.318
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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