› 6t2v

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the RecBCD in complex with Chi-plus2 substrate

Released:
DOI: 10.2210/pdb6t2v/pdb
PDB entry 6t2v coloured by chain, front view.
PDB entry 6t2v coloured by chain, side view.
PDB entry 6t2v coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair.
Cheng K, Wilkinson M, Chaban Y, Wigley DB
Nat Struct Mol Biol 27 71-77 (2020)
PMID: 31907455
Related structures: EMD-10370

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120626 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
RecBCD enzyme subunit RecB Chain: B
Molecule details ›
Chain: B
Length: 1181 amino acids
Theoretical weight: 134.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08394 (Residues: 1-1180; Coverage: 100%)
Gene names: JW2788, b2820, ior, recB, rorA
Sequence domains:
RecBCD enzyme subunit RecC Chain: C
Molecule details ›
Chain: C
Length: 1122 amino acids
Theoretical weight: 128.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07648 (Residues: 1-1122; Coverage: 100%)
Gene names: JW2790, b2822, recC
Sequence domains:
RecBCD enzyme subunit RecD Chain: D
Molecule details ›
Chain: D
Length: 608 amino acids
Theoretical weight: 66.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04993 (Residues: 1-608; Coverage: 100%)
Gene names: JW2787, b2819, hopE, recD
Sequence domains:
DNA (Chi-plus2) Chain: X
Molecule details ›
Chain: X
Length: 87 nucleotides
Theoretical weight: 26.77 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-10370
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Single-molecule insight into stalled replication fork rescue in Escherichia coli.
Bianco et al. (2021)
2 more

2 mentions without citation

A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair.
Cheng et al. (2020)
1 more