› 6t6h

X-ray diffraction
1.18Å resolution

Apo structure of the Bottromycin epimerase BotH

Released:
DOI: 10.2210/pdb6t6h/pdb
PDB entry 6t6h coloured by chain, front view.
PDB entry 6t6h coloured by chain, side view.
PDB entry 6t6h coloured by chain, top view.
Source organism: Streptomyces sp. BC16019
Primary publication:
The bottromycin epimerase BotH defines a group of atypical α/β-hydrolase-fold enzymes.
Sikandar A, Franz L, Adam S, Santos-Aberturas J, Horbal L, Luzhetskyy A, Truman AW, Kalinina OV, Koehnke J
Nat Chem Biol 16 1013-1018 (2020)
PMID: 32601484

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 33.09 KDa
Source organism: Streptomyces sp. BC16019
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K4MHV9 (Residues: 2-293; Coverage: 100%)
Gene name: botH
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I222
Unit cell:
a: 66.683Å b: 80.133Å c: 88.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.157 0.168
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Cytochromes P450 Associated with the Biosyntheses of Ribosomally Synthesized and Post-translationally Modified Peptides.
Zhong G. (2023)
2 more