› 6ta6

Electron Microscopy
3.2Å resolution

MexAB assembly of the Pseudomonas MexAB-OprM efflux pump reconstituted in nanodiscs

Released:
DOI: 10.2210/pdb6ta6/pdb
PDB entry 6ta6 coloured by chain, front view.
PDB entry 6ta6 coloured by chain, side view.
PDB entry 6ta6 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.
Glavier M, Puvanendran D, Salvador D, Decossas M, Phan G, Garnier C, Frezza E, Cece Q, Schoehn G, Picard M, Taveau JC, Daury L, Broutin I, Lambert O
Nat Commun 11 4948 (2020)
PMID: 33009415
Related structures: EMD-10395

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-156448 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Outer membrane protein OprM Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 474 amino acids
Theoretical weight: 51.74 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51487 (Residues: 18-485; Coverage: 100%)
Gene names: PA0427, oprK, oprM
Sequence domains: Outer membrane efflux protein
Multidrug resistance protein MexA Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 366 amino acids
Theoretical weight: 39.46 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P52477 (Residues: 24-383; Coverage: 100%)
Gene names: PA0425, mexA
Sequence domains:
Multidrug resistance protein MexB Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 1052 amino acids
Theoretical weight: 113.71 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P52002 (Residues: 1-1046; Coverage: 100%)
Gene names: PA0426, mexB
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-10395
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Molecular mechanisms of antibiotic resistance revisited.
Darby et al. (2023)
11 more

8 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
7 more