› 6taa

X-ray diffraction
2.1Å resolution

STRUCTURE AND MOLECULAR MODEL REFINEMENT OF ASPERGILLUS ORYZAE (TAKA) ALPHA-AMYLASE: AN APPLICATION OF THE SIMULATED-ANNEALING METHOD

Released:
DOI: 10.2210/pdb6taa/pdb
PDB entry 6taa coloured by chain, front view.
PDB entry 6taa coloured by chain, side view.
PDB entry 6taa coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Structure and molecular model refinement of Aspergillus oryzae (TAKA) alpha-amylase: an application of the simulated-annealing method.
Swift HJ, Brady L, Derewenda ZS, Dodson EJ, Dodson GG, Turkenburg JP, Wilkinson AJ
Acta Crystallogr B 47 ( Pt 4) 535-44 (1991)
PMID: 1930835

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase A type-1/2 Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 52.53 KDa
Source organism: Aspergillus oryzae
Expression system: Not provided
UniProt:
  • Canonical: P0C1B3 (Residues: 22-499; Coverage: 100%)
Gene names: AO090023000944, AO090120000196, Taa-G1, Taa-G2, amy1, amy2, amyI, amyII
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 51.04Å b: 67.183Å c: 133.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

Characterized allergens causing bakers' asthma.
Baur et al. (1998)
3 more

10 mentions without citation

Side-chain flexibility in protein-ligand binding: the minimal rotation hypothesis.
Zavodszky et al. (2005)
9 more