› 6tky

X-ray diffraction
2.55Å resolution

Crystal structure of the DHR2 domain of DOCK10 in complex with CDC42

Released:
DOI: 10.2210/pdb6tky/pdb
PDB entry 6tky coloured by chain, front view.
PDB entry 6tky coloured by chain, side view.
PDB entry 6tky coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for CDC42 and RAC activation by the dual specificity GEF DOCK10
Fan D, Yang J, Cronin N, Barford D
Biorxiv (2022)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158141 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dedicator of cytokinesis protein 10 Chain: B
Molecule details ›
Chain: B
Length: 457 amino acids
Theoretical weight: 53.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY6 (Residues: 1694-2150; Coverage: 21%)
Gene names: DOCK10, KIAA0694, ZIZ3
Sequence domains:
Cell division control protein 42 homolog Chains: C, D
Molecule details ›
Chains: C, D
Length: 188 amino acids
Theoretical weight: 20.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60953 (Residues: 1-188; Coverage: 98%)
Gene name: CDC42
Sequence domains: Ras family
Dedicator of cytokinesis protein 10 Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 53.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY6 (Residues: 1694-2151; Coverage: 21%)
Gene names: DOCK10, KIAA0694, ZIZ3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P41212
Unit cell:
a: 96.898Å b: 96.898Å c: 310.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.246
Expression system: Escherichia coli

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