› 6tkz

X-ray diffraction
2.64Å resolution

Crystal structure of the DHR2 domain of DOCK10 in complex with CDC42

Released:
DOI: 10.2210/pdb6tkz/pdb
PDB entry 6tkz coloured by chain, front view.
PDB entry 6tkz coloured by chain, side view.
PDB entry 6tkz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for CDC42 and RAC activation by the dual specificity GEF DOCK10
Fan D, Yang J, Cronin N, Barford D
Biorxiv (2022)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158152 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dedicator of cytokinesis protein 10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 53.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY6 (Residues: 1694-2151; Coverage: 21%)
Gene names: DOCK10, KIAA0694, ZIZ3
Sequence domains:
Cell division control protein 42 homolog Chains: C, D
Molecule details ›
Chains: C, D
Length: 188 amino acids
Theoretical weight: 20.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60953 (Residues: 1-188; Coverage: 98%)
Gene name: CDC42
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41212
Unit cell:
a: 97.17Å b: 97.17Å c: 312Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.274
Expression system: Escherichia coli

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