› 6tm1

X-ray diffraction
3.71Å resolution

Crystal structure of the DHR2 domain of DOCK10 in complex with RAC3

Released:
DOI: 10.2210/pdb6tm1/pdb
PDB entry 6tm1 coloured by chain, front view.
PDB entry 6tm1 coloured by chain, side view.
PDB entry 6tm1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for CDC42 and RAC activation by the dual specificity GEF DOCK10
Fan D, Yang J, Cronin N, Barford D
Biorxiv (2022)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158090 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dedicator of cytokinesis protein 10 Chain: B
Molecule details ›
Chain: B
Length: 457 amino acids
Theoretical weight: 53.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY6 (Residues: 1694-2150; Coverage: 21%)
Gene names: DOCK10, KIAA0694, ZIZ3
Sequence domains:
Ras-related C3 botulinum toxin substrate 3 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60763 (Residues: 1-192; Coverage: 100%)
Gene name: RAC3
Sequence domains: Ras family
Dedicator of cytokinesis protein 10 Chain: C
Molecule details ›
Chain: C
Length: 458 amino acids
Theoretical weight: 53.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY6 (Residues: 1694-2151; Coverage: 21%)
Gene names: DOCK10, KIAA0694, ZIZ3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I222
Unit cell:
a: 110.079Å b: 128.455Å c: 215.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.26 0.321
Expression system: Escherichia coli

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