› 6tqo

Electron Microscopy
3.8Å resolution

rrn anti-termination complex

Released:
DOI: 10.2210/pdb6tqo/pdb
PDB entry 6tqo coloured by chain, front view.
PDB entry 6tqo coloured by chain, side view.
PDB entry 6tqo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis.
Huang YH, Hilal T, Loll B, Bürger J, Mielke T, Böttcher C, Said N, Wahl MC
Mol Cell 79 1024-1036.e5 (2020)
PMID: 32871103
Related structures: EMD-10548

Function and Biology Details

Reactions catalysed: 
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120654 (preferred)
Entry contents:
11 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (14 distinct):
Nus factor SuhB Chain: T
Molecule details ›
Chain: T
Length: 267 amino acids
Theoretical weight: 29.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADG4 (Residues: 1-263; Coverage: 99%)
Gene names: JW2517, b2533, ssyA, suhB
Sequence domains: Inositol monophosphatase family
Nus factor SuhB Chain: S
Molecule details ›
Chain: S
Length: 271 amino acids
Theoretical weight: 29.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADG4 (Residues: 1-267; Coverage: 100%)
Gene names: JW2517, b2533, ssyA, suhB
Sequence domains: Inositol monophosphatase family
Transcription termination/antitermination protein NusA Chain: A
Molecule details ›
Chain: A
Length: 497 amino acids
Theoretical weight: 55.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFF6 (Residues: 1-495; Coverage: 100%)
Gene names: JW3138, b3169, nusA
Sequence domains:
Transcription antitermination protein NusB Chain: B
Molecule details ›
Chain: B
Length: 141 amino acids
Theoretical weight: 15.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A780 (Residues: 1-139; Coverage: 100%)
Gene names: JW0406, b0416, groNB, nusB, ssyB
Sequence domains: NusB family
Small ribosomal subunit protein uS10 Chain: E
Molecule details ›
Chain: E
Length: 106 amino acids
Theoretical weight: 12.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Transcription termination/antitermination protein NusG Chain: G
Molecule details ›
Chain: G
Length: 184 amino acids
Theoretical weight: 20.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFG0 (Residues: 1-181; Coverage: 100%)
Gene names: JW3945, b3982, nusG
Sequence domains:
DNA-directed RNA polymerase subunit alpha Chains: U, V
Molecule details ›
Chains: U, V
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: W
Molecule details ›
Chain: W
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit beta Chain: X
Molecule details ›
Chain: X
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: Y
Molecule details ›
Chain: Y
Length: 1417 amino acids
Theoretical weight: 156.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
Small ribosomal subunit protein uS4 Chain: C
Molecule details ›
Chain: C
Length: 208 amino acids
Theoretical weight: 23.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
tDNA Chain: L
Molecule details ›
Chain: L
Length: 35 nucleotides
Theoretical weight: 10.65 KDa
ntDNA Chain: K
Molecule details ›
Chain: K
Length: 35 nucleotides
Theoretical weight: 10.82 KDa
rrnGnut RNA Chain: R
Molecule details ›
Chain: R
Length: 85 nucleotides
Theoretical weight: 27.44 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-10548
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Bacterial transcription during growth arrest.
Bergkessel M. (2021)
3 more

3 mentions without citation

The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.
Gerovac et al. (2021)
2 more