Function and Biology Details
Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-124769 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
F-box/WD repeat-containing protein 1A
Molecule details ›
S-phase kinase-associated protein 1
Molecule details ›
Cullin-1
Molecule details ›
Chain: C
Length: 776 amino acids
Theoretical weight: 89.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 776 amino acids
Theoretical weight: 89.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q13616 (Residues: 1-776; Coverage: 100%)
Sequence domains:
NEDD8
Molecule details ›
Chain: N
Length: 77 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
Sequence domains: Ubiquitin family
Length: 77 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
- Canonical: Q15843 (Residues: 1-76; Coverage: 94%)
Sequence domains: Ubiquitin family
RING-type domain-containing protein
Molecule details ›
Chain: R
Length: 108 amino acids
Theoretical weight: 12.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: RING-H2 zinc finger domain
Length: 108 amino acids
Theoretical weight: 12.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: H2QLR9 (Residues: 1-108; Coverage: 100%)
Sequence domains: RING-H2 zinc finger domain
Ubiquitin-conjugating enzyme E2 D2
Molecule details ›
Chain: D
Length: 147 amino acids
Theoretical weight: 16.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Ubiquitin-conjugating enzyme
Length: 147 amino acids
Theoretical weight: 16.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P62837 (Residues: 1-147; Coverage: 100%)
Sequence domains: Ubiquitin-conjugating enzyme
Ubiquitin
Molecule details ›
Chain: U
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Ubiquitin family
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Sequence domains: Ubiquitin family
NF-kappa-B inhibitor alpha
