› 6u2k

Electron Microscopy
2.93Å resolution

EM structure of MPEG-1 (L425K, alpha conformation) soluble pre-pore complex

Released:
DOI: 10.2210/pdb6u2k/pdb
PDB entry 6u2k coloured by chain, front view.
PDB entry 6u2k coloured by chain, side view.
PDB entry 6u2k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1.
Pang SS, Bayly-Jones C, Radjainia M, Spicer BA, Law RHP, Hodel AW, Parsons ES, Ekkel SM, Conroy PJ, Ramm G, Venugopal H, Bird PI, Hoogenboom BW, Voskoboinik I, Gambin Y, Sierecki E, Dunstone MA, Whisstock JC
Nat Commun 10 4288 (2019)
PMID: 31537793
Related structures: EMD-20621

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Macrophage-expressed gene 1 protein Chain: B
Molecule details ›
Chain: B
Length: 642 amino acids
Theoretical weight: 71.13 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q2M385 (Residues: 18-653; Coverage: 91%)
Gene name: MPEG1
Sequence domains: MAC/Perforin domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-20621
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

To Kill But Not Be Killed: Controlling the Activity of Mammalian Pore-Forming Proteins.
Krawczyk et al. (2020)
10 more

2 mentions without citation

Ancient but Not Forgotten: New Insights Into MPEG1, a Macrophage Perforin-Like Immune Effector.
Bayly-Jones et al. (2020)
1 more