› 6u8z

X-ray diffraction
1.8Å resolution

Crystal Structure of Catalytic Domain of Human Phospholipase D1

Released:
DOI: 10.2210/pdb6u8z/pdb
PDB entry 6u8z coloured by chain, front view.
PDB entry 6u8z coloured by chain, side view.
PDB entry 6u8z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human PLD1 provides insight into activation by PI(4,5)P2 and RhoA.
Bowling FZ, Salazar CM, Bell JA, Huq TS, Frohman MA, Airola MV
Nat Chem Biol 16 400-407 (2020)
PMID: 32198492

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = choline + a phosphatidate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase D1 Chain: A
Molecule details ›
Chain: A
Length: 603 amino acids
Theoretical weight: 69.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13393 (Residues: 330-1074; Coverage: 56%)
Gene name: PLD1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X17B1
Spacegroup: P41212
Unit cell:
a: 75.209Å b: 75.209Å c: 239.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.214
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Regulatory mechanisms triggered by enzyme interactions with lipid membrane surfaces.
Yu et al. (2023)
16 other articles

6 mentions without citation

Structure and regulation of human phospholipase D.
Bowling et al. (2021)
5 more