› 6ueu

X-ray diffraction
1.8Å resolution

Crystal structure of BF DNA polymerase F710Y mutant bound to tetrahydrofuran and dATP

Released:
DOI: 10.2210/pdb6ueu/pdb
PDB entry 6ueu coloured by chain, front view.
PDB entry 6ueu coloured by chain, side view.
PDB entry 6ueu coloured by chain, top view.
Source organisms:
Entry authors: Wu EY, Beese LS

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112997 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase I Chains: A, D
Molecule details ›
Chains: A, D
Length: 580 amino acids
Theoretical weight: 66.14 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: D9N168 (Residues: 1-579; Coverage: 100%)
Gene names: DPO1, polA
Sequence domains: DNA polymerase family A
DNA (5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(2DT))-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 9 nucleotides
Theoretical weight: 2.7 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*(3DR)P*AP*CP*GP*TP*GP*AP*TP*CP*G)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 12 nucleotides
Theoretical weight: 3.55 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
3 bound ligands:
Ligand DTP 2 x DTP
Ligand SO4 1 x SO4
Ligand MPD 1 x MPD
1 modified residue:
Ligand 2DT 2 x 2DT

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 93.875Å b: 109.068Å c: 150.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.242
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

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