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› 6ulz

X-ray diffraction
3.1Å resolution

Adenylation domain of the initiation module of LgrA mutant P483M

Released:
DOI: 10.2210/pdb6ulz/pdb
Model geometry
Fit model/data
PDB entry 6ulz coloured by chain, front view.
PDB entry 6ulz coloured by chain, side view.
PDB entry 6ulz coloured by chain, top view.
Source organism: Brevibacillus parabrevis
Primary publication:
Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
Alonzo DA, Chiche-Lapierre C, Tarry MJ, Wang J, Schmeing TM
Nat Chem Biol 16 493-496 (2020)
PMID: 32066969

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Linear gramicidin synthase subunit A Chain: A
Molecule details ›
Chain: A
Length: 687 amino acids
Theoretical weight: 77.75 KDa
Source organism: Brevibacillus parabrevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70LM7 (Residues: 2-684; Coverage: 30%)
Gene name: lgrA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand FMT 3 x FMT
Ligand APC 1 x APC
Ligand KIV 1 x KIV
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 161.18Å b: 161.18Å c: 138.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Biosynthesis of depsipeptides, or Depsi: The peptides with varied generations.
Alonzo et al. (2020)
3 more

1 mention without citation

Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases.
Patel et al. (2023)