› 6una

X-ray diffraction
2.55Å resolution

Crystal structure of inactive p38gamma

Released:
DOI: 10.2210/pdb6una/pdb
PDB entry 6una coloured by chain, front view.
PDB entry 6una coloured by chain, side view.
PDB entry 6una coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Dynamic Switch in Inactive p38γ Leads to an Excited State on the Pathway to an Active Kinase.
Aoto PC, Stanfield RL, Wilson IA, Dyson HJ, Wright PE
Biochemistry 58 5160-5172 (2019)
PMID: 31794659

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53778 (Residues: 7-367; Coverage: 98%)
Gene names: ERK6, MAPK12, SAPK3
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 47.032Å b: 66.359Å c: 68.568Å
α: 115.27° β: 102.49° γ: 96.83°
R-values:
R R work R free
0.198 0.195 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Spotlight on the Ballet of Proteins: The Structural Dynamic Properties of Proteins Illuminated by Solution NMR.
Tokunaga et al. (2020)
2 other articles

3 mentions without citation

A Dynamic Switch in Inactive p38γ Leads to an Excited State on the Pathway to an Active Kinase.
Aoto et al. (2019)
2 more