6uui

X-ray diffraction
2.07Å resolution

Crystal structure of the heterocomplex between coil 2B domains of wild-type keratin 1 (KRT1) and keratin 10 (KRT10) containing mutation Cys401Ala

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-137542 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Keratin, type II cytoskeletal 1 Chain: C
Molecule details ›
Chain: C
Length: 120 amino acids
Theoretical weight: 13.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04264 (Residues: 370-489; Coverage: 19%)
Gene names: KRT1, KRTA
Sequence domains: Intermediate filament protein
Keratin, type I cytoskeletal 10 Chain: X
Molecule details ›
Chain: X
Length: 122 amino acids
Theoretical weight: 14.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13645 (Residues: 337-456; Coverage: 21%)
Gene names: KPP, KRT10
Sequence domains: Intermediate filament protein

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6122
Unit cell:
a: 100.124Å b: 100.124Å c: 224.788Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.25 0.277
Expression system: Escherichia coli BL21(DE3)