› 6uym

X-ray diffraction
2.85Å resolution

Structure of Hepatitis C Virus Envelope Glycoprotein E2mc3-v6 redesigned core from genotype 1a bound to broadly neutralizing antibody AR3C

Released:
DOI: 10.2210/pdb6uym/pdb
PDB entry 6uym coloured by chain, front view.
PDB entry 6uym coloured by chain, side view.
PDB entry 6uym coloured by chain, top view.
Source organisms:
Primary publication:
Proof of concept for rational design of hepatitis C virus E2 core nanoparticle vaccines.
He L, Tzarum N, Lin X, Shapero B, Sou C, Mann CJ, Stano A, Zhang L, Nagy K, Giang E, Law M, Wilson IA, Zhu J
Sci Adv 6 eaaz6225 (2020)
PMID: 32494617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-231855 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope glycoprotein E2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 176 amino acids
Theoretical weight: 19.29 KDa
Source organism: Hepatitis C virus (isolate H)
Expression system: Homo sapiens
Fab AR3C heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 233 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab AR3C light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 214 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 45.101Å b: 90.84Å c: 94.033Å
α: 84.5° β: 78.11° γ: 77.09°
R-values:
R R work R free
0.217 0.214 0.26
Expression system: Homo sapiens

Quick links

Citations

12 review citations

Emerging Concepts and Technologies in Vaccine Development.
Brisse et al. (2020)
11 more

1 mention without citation

A conserved epitope III on hepatitis C virus E2 protein has alternate conformations facilitating cell binding or virus neutralization.
Deng et al. (2021)