› 6v22

Electron Microscopy
3.2Å resolution

Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex

Released:
DOI: 10.2210/pdb6v22/pdb
PDB entry 6v22 coloured by chain, front view.
PDB entry 6v22 coloured by chain, side view.
PDB entry 6v22 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular structures of the human Slo1 K+ channel in complex with β4.
Tao X, MacKinnon R
Elife 8 (2019)
PMID: 31815672
Related structures: EMD-21025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171394 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium-activated potassium channel subunit alpha-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1065 amino acids
Theoretical weight: 119.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q12791 (Residues: 66-1179; Coverage: 85%)
Gene names: KCNMA, KCNMA1, SLO
Sequence domains:
Calcium-activated potassium channel subunit beta-4 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 219 amino acids
Theoretical weight: 24.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q86W47 (Residues: 1-210; Coverage: 100%)
Gene name: KCNMB4
Sequence domains: Calcium-activated potassium channel, beta subunit

Ligands and Environments

6 bound ligands:
Ligand MG 4 x MG
Ligand CA 8 x CA
Ligand POV 60 x POV
Ligand CLR 16 x CLR
Ligand K 4 x K
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-21025
Expression system: Homo sapiens

Quick links

Citations

10 review citations

Ion channels and channelopathies in glomeruli.
Staruschenko et al. (2023)
9 more

11 mentions without citation

Structural and Functional Coupling of Calcium-Activated BK Channels and Calcium-Permeable Channels Within Nanodomain Signaling Complexes.
Shah et al. (2021)
10 more