› 6v9i

Electron Microscopy
5.2Å resolution

cryo-EM structure of Cullin5 bound to RING-box protein 2 (Cul5-Rbx2)

Released:
DOI: 10.2210/pdb6v9i/pdb
PDB entry 6v9i coloured by chain, front view.
PDB entry 6v9i coloured by chain, side view.
PDB entry 6v9i coloured by chain, top view.
Source organisms:
Primary publication:
Structure and dynamics of the ASB9 CUL-RING E3 Ligase.
Lumpkin RJ, Baker RW, Leschziner AE, Komives EA
Nat Commun 11 2866 (2020)
PMID: 32513959
Related structures: EMD-21121

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139120 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cullin-5; Immunoglobulin G-binding protein G Chain: C
Molecule details ›
Chain: C
Length: 863 amino acids
Theoretical weight: 100.46 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06654 (Residues: 229-282; Coverage: 13%)
  • Canonical: Q93034 (Residues: 2-780; Coverage: 100%)
Gene names: CUL5, VACM1, spg
Sequence domains:
RING-box protein 2 Chain: R
Molecule details ›
Chain: R
Length: 113 amino acids
Theoretical weight: 12.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WTZ1 (Residues: 1-113; Coverage: 100%)
Gene names: Rbx2, Rnf7, Sag
Sequence domains: RING-H2 zinc finger domain

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.2Å
Relevant EMDB volumes: EMD-21121
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

2 review citations

Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems.
James et al. (2022)
1 more

3 mentions without citation

Structure and dynamics of the ASB9 CUL-RING E3 Ligase.
Lumpkin et al. (2020)
2 more