› 6vcb

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the Glucagon-like peptide-1 receptor in complex with G protein, GLP-1 peptide and a positive allosteric modulator

Released:
DOI: 10.2210/pdb6vcb/pdb
PDB entry 6vcb coloured by chain, front view.
PDB entry 6vcb coloured by chain, side view.
PDB entry 6vcb coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into probe-dependent positive allosterism of the GLP-1 receptor.
Bueno AB, Sun B, Willard FS, Feng D, Ho JD, Wainscott DB, Showalter AD, Vieth M, Chen Q, Stutsman C, Chau B, Ficorilli J, Agejas FJ, Cumming GR, Jiménez A, Rojo I, Kobilka TS, Kobilka BK, Sloop KW
Nat Chem Biol 16 1105-1110 (2020)
PMID: 32690941
Related structures: EMD-21147

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208382 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Glucagon-like peptide 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 445 amino acids
Theoretical weight: 51.23 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P43220 (Residues: 24-422; Coverage: 91%)
Gene name: GLP1R
Sequence domains:
Glucagon-like peptide 1(7-37) Chain: P
Molecule details ›
Chain: P
Length: 31 amino acids
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01275 (Residues: 98-128; Coverage: 19%)
Gene name: GCG
Sequence domains: Peptide hormone
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 160 amino acids
Theoretical weight: 17.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand QW7 1 x QW7
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-21147
Expression systems:
  • Not provided
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

2 review citations

Allostery: Allosteric Cancer Drivers and Innovative Allosteric Drugs.
Nussinov et al. (2022)
1 more

19 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
18 more