› 6vja

Electron Microscopy
3.3Å resolution

Structure of CD20 in complex with rituximab Fab

Released:
DOI: 10.2210/pdb6vja/pdb
PDB entry 6vja coloured by chain, front view.
PDB entry 6vja coloured by chain, side view.
PDB entry 6vja coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of CD20 in complex with the therapeutic monoclonal antibody rituximab.
Rougé L, Chiang N, Steffek M, Kugel C, Croll TI, Tam C, Estevez A, Arthur CP, Koth CM, Ciferri C, Kraft E, Payandeh J, Nakamura G, Koerber JT, Rohou A
Science 367 1224-1230 (2020)
PMID: 32079680
Related structures: EMD-21212

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210511 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
B-lymphocyte antigen CD20 Chains: C, D
Molecule details ›
Chains: C, D
Length: 278 amino acids
Theoretical weight: 31.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P11836 (Residues: 41-297; Coverage: 87%)
Gene names: CD20, MS4A1
Sequence domains: CD20-like family
Rituximab Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 224 amino acids
Theoretical weight: 23.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Rituximab Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 213 amino acids
Theoretical weight: 23.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand Y01 6 x Y01
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-21212
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

21 review citations

The Role of Complement in the Mechanism of Action of Therapeutic Anti-Cancer mAbs.
Golay et al. (2020)
20 more

3 mentions without citation

Drug discovery in the era of cryo-electron microscopy.
Robertson et al. (2022)
2 more