› 6vr4

X-ray diffraction
3.5Å resolution

Virion-packaged DNA-dependent RNA polymerase of crAss-like phage phi14:2

Released:
DOI: 10.2210/pdb6vr4/pdb
PDB entry 6vr4 coloured by chain, front view.
PDB entry 6vr4 coloured by chain, side view.
PDB entry 6vr4 coloured by chain, top view.
Source organism: Cellulophaga phage phi14:2
Primary publication:
Structure and function of virion RNA polymerase of a crAss-like phage.
Drobysheva AV, Panafidina SA, Kolesnik MV, Klimuk EI, Minakhin L, Yakunina MV, Borukhov S, Nilsson E, Holmfeldt K, Yutin N, Makarova KS, Koonin EV, Severinov KV, Leiman PG, Sokolova ML
Nature 589 306-309 (2021)
PMID: 33208949

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Structural protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 2194 amino acids
Theoretical weight: 251.18 KDa
Source organism: Cellulophaga phage phi14:2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S0A2C3 (Residues: 1-2180; Coverage: 100%)
Gene name: Phi14:2_gp077

Ligands and Environments

2 bound ligands:
Ligand CL 21 x CL
Ligand NA 6 x NA
1 modified residue:
Ligand MSE 78 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 266.441Å b: 297.181Å c: 92.015Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Mechanisms and clinical importance of bacteriophage resistance.
Egido et al. (2022)
2 more

8 mentions without citation

Implications of AlphaFold2 for crystallographic phasing by molecular replacement.
McCoy et al. (2022)
7 more