› 6vvo

Electron Microscopy
3.4Å resolution

Structure of the human clamp loader (Replication Factor C, RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen, PCNA)

Released:
DOI: 10.2210/pdb6vvo/pdb
PDB entry 6vvo coloured by chain, front view.
PDB entry 6vvo coloured by chain, side view.
PDB entry 6vvo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human clamp loader bound to the sliding clamp: a further twist on AAA+ mechanism
Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA.
(2020)
Related structures: EMD-21405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146044 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Replication factor C subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 66.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35251 (Residues: 556-1148; Coverage: 52%)
Gene names: RFC1, RFC140
Sequence domains:
Replication factor C subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 39.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35250 (Residues: 1-354; Coverage: 100%)
Gene name: RFC2
Sequence domains:
Replication factor C subunit 5 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 38.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40937 (Residues: 1-340; Coverage: 100%)
Gene name: RFC5
Sequence domains:
Replication factor C subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 363 amino acids
Theoretical weight: 39.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35249 (Residues: 1-363; Coverage: 100%)
Gene name: RFC4
Sequence domains:
Replication factor C subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 356 amino acids
Theoretical weight: 40.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40938 (Residues: 1-356; Coverage: 100%)
Gene name: RFC3
Sequence domains: DNA polymerase III, delta subunit
Proliferating cell nuclear antigen Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand AGS 4 x AGS
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-21405
Expression system: Escherichia coli

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