› 6vw8

X-ray diffraction
2.3Å resolution

Formate Dehydrogenase FdsABG subcomplex FdsBG from C. necator

Released:
DOI: 10.2210/pdb6vw8/pdb
PDB entry 6vw8 coloured by chain, front view.
PDB entry 6vw8 coloured by chain, side view.
PDB entry 6vw8 coloured by chain, top view.
Source organism: Cupriavidus necator
Primary publication:
Crystallographic and kinetic analyses of the FdsBG subcomplex of the cytosolic formate dehydrogenase FdsABG from Cupriavidus necator.
Young T, Niks D, Hakopian S, Tam TK, Yu X, Hille R, Blaha GM
J Biol Chem 295 6570-6585 (2020)
PMID: 32249211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170981 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent formate dehydrogenase gamma subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 215 amino acids
Theoretical weight: 23.37 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q0KDY3 (Residues: 2-176; Coverage: 99%)
Gene names: E6A55_03230, H16_A0640, fdsG
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 520 amino acids
Theoretical weight: 55.29 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q0KDY2 (Residues: 1-520; Coverage: 100%)
Gene names: E6A55_03235, H16_A0641, fdsB
Sequence domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
4 bound ligands:
Ligand FES 2 x FES
Ligand K 2 x K
Ligand SF4 2 x SF4
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1, APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 132.656Å b: 127.05Å c: 99.84Å
α: 90° β: 123.5° γ: 90°
R-values:
R R work R free
0.183 0.182 0.223
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

2 review citations

Spectroscopic Studies of Mononuclear Molybdenum Enzyme Centers.
Kirk et al. (2022)
1 more

1 mention without citation

Crystallographic and kinetic analyses of the FdsBG subcomplex of the cytosolic formate dehydrogenase FdsABG from Cupriavidus necator.
Young et al. (2020)