› 6wbv

Electron Microscopy
2.5Å resolution

Structure of human ferroportin bound to hepcidin and cobalt in lipid nanodisc

Released:
DOI: 10.2210/pdb6wbv/pdb
PDB entry 6wbv coloured by chain, front view.
PDB entry 6wbv coloured by chain, side view.
PDB entry 6wbv coloured by chain, top view.
Source organisms:
Primary publication:
Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms.
Billesbølle CB, Azumaya CM, Kretsch RC, Powers AS, Gonen S, Schneider S, Arvedson T, Dror RO, Cheng Y, Manglik A
Nature 586 807-811 (2020)
PMID: 32814342
Related structures: EMD-21599

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-211528 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hepcidin-25 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.8 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P81172 (Residues: 60-84; Coverage: 42%)
Gene names: HAMP, HEPC, LEAP1, UNQ487/PRO1003
Sequence domains: Hepcidin
Solute carrier family 40 member 1 Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 66.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NP59 (Residues: 1-571; Coverage: 100%)
Gene names: FPN, FPN1, IREG1, MSTP079, SLC11A3, SLC40A1
Sequence domains: Ferroportin1 (FPN1)
Fab45D8 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 24.01 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Fab45D8 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.85 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand AGA 2 x AGA
Ligand CO 2 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-21599
Expression systems:
  • Not provided
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

40 review citations

Hepcidin-Ferroportin Interaction Controls Systemic Iron Homeostasis.
Nemeth et al. (2021)
39 more

7 mentions without citation

Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms.
Billesbølle et al. (2020)
6 more