› 6wha

Electron Microscopy
3.36Å resolution

HTR2A bound to 25-CN-NBOH in complex with a mini-Galpha-q protein, beta/gamma subunits and an active-state stabilizing single-chain variable fragment (scFv16) obtained by cryo-electron microscopy (cryoEM)

Released:
DOI: 10.2210/pdb6wha/pdb
PDB entry 6wha coloured by chain, front view.
PDB entry 6wha coloured by chain, side view.
PDB entry 6wha coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a Hallucinogen-Activated Gq-Coupled 5-HT2A Serotonin Receptor
Kim K, Che T, Panova O, Shoichet BK, Skiniotis G, Roth BL
Cell 182 1574-1588 (2020)
Related structures: EMD-21669

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209526 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
5-hydroxytryptamine receptor 2A; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 497 amino acids
Theoretical weight: 56.17 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P28223 (Residues: 66-404; Coverage: 72%)
Gene names: HTR2, HTR2A, cybC
Sequence domains:
G subunit q (Gi2-mini-Gq chimeric) Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
single Fab chain (svFv16) Chain: E
Molecule details ›
Chain: E
Length: 267 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand U0G 1 x U0G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.36Å
Relevant EMDB volumes: EMD-21669
Expression system: Spodoptera frugiperda

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