› 6wpw

Electron Microscopy
3.1Å resolution

GCGR-Gs signaling complex bound to a designed glucagon derivative

Released:
DOI: 10.2210/pdb6wpw/pdb
PDB entry 6wpw coloured by chain, front view.
PDB entry 6wpw coloured by chain, side view.
PDB entry 6wpw coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into differences in G protein activation by family A and family B GPCRs.
Hilger D, Kumar KK, Hu H, Pedersen MF, O'Brien ES, Giehm L, Jennings C, Eskici G, Inoue A, Lerch M, Mathiesen JM, Skiniotis G, Kobilka BK
Science 369 (2020)
PMID: 32732395
Related structures: EMD-21866

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211259 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: D
Molecule details ›
Chain: D
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nb35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Glucagon derivative ZP3780 Chain: P
Molecule details ›
Chain: P
Length: 29 amino acids
Theoretical weight: 3.46 KDa
Source organism: synthetic construct
Expression system: Not provided
Glucagon receptor Chain: R
Molecule details ›
Chain: R
Length: 496 amino acids
Theoretical weight: 56.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47871 (Residues: 27-477; Coverage: 100%)
Gene name: GCGR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-21866
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

7 review citations

Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors.
Zhou et al. (2021)
6 more

14 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
13 more