› 6wth

Electron Microscopy
3.06Å resolution

Full-length human ENaC ECD

Released:
DOI: 10.2210/pdb6wth/pdb
PDB entry 6wth coloured by chain, front view.
PDB entry 6wth coloured by chain, side view.
PDB entry 6wth coloured by chain, top view.
Source organisms:
Primary publication:
Molecular principles of assembly, activation, and inhibition in epithelial sodium channel.
Noreng S, Posert R, Bharadwaj A, Houser A, Baconguis I
Elife 9 (2020)
PMID: 32729833
Related structures: EMD-21896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-153348 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Amiloride-sensitive sodium channel subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 669 amino acids
Theoretical weight: 75.78 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P37088 (Residues: 1-669; Coverage: 100%)
Gene names: SCNN1, SCNN1A
Sequence domains: Amiloride-sensitive sodium channel
Amiloride-sensitive sodium channel subunit beta Chain: B
Molecule details ›
Chain: B
Length: 640 amino acids
Theoretical weight: 72.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51168 (Residues: 1-640; Coverage: 100%)
Gene name: SCNN1B
Sequence domains: Amiloride-sensitive sodium channel
Amiloride-sensitive sodium channel subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 649 amino acids
Theoretical weight: 74.35 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51170 (Residues: 1-649; Coverage: 100%)
Gene name: SCNN1G
Sequence domains: Amiloride-sensitive sodium channel
7B1 Fab Chains: D, E
Molecule details ›
Chains: D, E
Length: 118 amino acids
Theoretical weight: 10.06 KDa
Source organism: Mus musculus
Expression system: Mus musculus
10D4 Fab Chains: F, G
Molecule details ›
Chains: F, G
Length: 115 amino acids
Theoretical weight: 9.81 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NA 1 x NA
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-21896
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

6 review citations

ENaC activation by proteases.
Anand et al. (2022)
5 more

7 mentions without citation

Accessibility of ENaC extracellular domain central core residues.
Zhang et al. (2022)
6 more