› 6wwz

Electron Microscopy
3.34Å resolution

Cryo-EM structure of the human chemokine receptor CCR6 in complex with CCL20 and a Go protein

Released:
DOI: 10.2210/pdb6wwz/pdb
PDB entry 6wwz coloured by chain, front view.
PDB entry 6wwz coloured by chain, side view.
PDB entry 6wwz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for chemokine receptor CCR6 activation by the endogenous protein ligand CCL20.
Wasilko DJ, Johnson ZL, Ammirati M, Che Y, Griffor MC, Han S, Wu H
Nat Commun 11 3031 (2020)
PMID: 32541785
Related structures: EMD-21950

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209407 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
C-C motif chemokine 20 Chain: C
Molecule details ›
Chain: C
Length: 70 amino acids
Theoretical weight: 8.04 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P78556 (Residues: 27-96; Coverage: 100%)
Gene names: CCL20, LARC, MIP3A, SCYA20
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Molecule details ›
Chain: Y
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09471 (Residues: 4-57, 182-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 287 amino acids
Theoretical weight: 30.9 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
C-C chemokine receptor type 6; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 529 amino acids
Theoretical weight: 60.06 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 22-127; Coverage: 99%)
  • Canonical: P51684 (Residues: 2-374; Coverage: 100%)
Gene names: CCR6, CKRL3, CMKBR6, GPR29, STRL22, cybC
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.34Å
Relevant EMDB volumes: EMD-21950
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Innate and Adaptive Immunity during SARS-CoV-2 Infection: Biomolecular Cellular Markers and Mechanisms.
Brown et al. (2023)
3 more

22 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
21 more