› 6wyh

X-ray diffraction
2.22Å resolution

Crystal structure of Human H-chain Ferritin variant 157C Delta C-star Modified with a RAFT Agent Soaked in an Acrylate Solution

Released:
DOI: 10.2210/pdb6wyh/pdb
PDB entry 6wyh coloured by chain, front view.
PDB entry 6wyh coloured by chain, side view.
PDB entry 6wyh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Anisotropic Dynamics and Mechanics of Macromolecular Crystals Containing Lattice-Patterned Polymer Networks.
Han K, Bailey JB, Zhang L, Tezcan FA
J Am Chem Soc 142 19402-19410 (2020)
PMID: 33124805

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 182 amino acids
Theoretical weight: 21.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 2-183; Coverage: 100%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain

Ligands and Environments

3 bound ligands:
Ligand FE 10 x FE
Ligand RFT 4 x RFT
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R32
Unit cell:
a: 128.88Å b: 128.88Å c: 291.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.193 0.245
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
2 other articles