› 6x70

X-ray diffraction
2.05Å resolution

Rev1-DNA Binary Complex

Released:
DOI: 10.2210/pdb6x70/pdb
PDB entry 6x70 coloured by chain, front view.
PDB entry 6x70 coloured by chain, side view.
PDB entry 6x70 coloured by chain, top view.
Source organisms:
Primary publication:
Visualizing Rev1 catalyze protein-template DNA synthesis.
Weaver TM, Cortez LM, Khoang TH, Washington MT, Agarwal PK, Freudenthal BD
Proc Natl Acad Sci U S A 117 25494-25504 (2020)
PMID: 32999062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120911 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 442 amino acids
Theoretical weight: 50.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P12689 (Residues: 305-746; Coverage: 45%)
Gene names: O6339, REV1, YOR346W
Sequence domains:
DNA (5'-D(*GP*GP*GP*GP*TP*GP*TP*GP*GP*TP*AP*G)-3') Chain: P
Molecule details ›
Chain: P
Length: 12 nucleotides
Theoretical weight: 3.81 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*CP*GP*CP*TP*AP*CP*CP*AP*CP*AP*CP*CP*CP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 17 nucleotides
Theoretical weight: 5.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 62.212Å b: 73.916Å c: 117.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.261
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Two-Metal-Ion Catalysis: Inhibition of DNA Polymerase Activity by a Third Divalent Metal Ion.
Wang et al. (2022)
5 more