› 6xpf

Electron Microscopy
5.9Å resolution

Cryo-EM structure of human ZnT8 WT, in the absence of zinc, determined in heterogeneous conformations- one subunit in an inward-facing and the other in an outward-facing conformation

Released:
DOI: 10.2210/pdb6xpf/pdb
PDB entry 6xpf coloured by chain, front view.
PDB entry 6xpf coloured by chain, side view.
PDB entry 6xpf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations.
Xue J, Xie T, Zeng W, Jiang Y, Bai XC
Elife 9 (2020)
PMID: 32723473
Related structures: EMD-22287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-coupled zinc antiporter SLC30A8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 35.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8IWU4 (Residues: 50-369; Coverage: 87%)
Gene names: SLC30A8, ZNT8
Sequence domains: Cation efflux family

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.9Å
Relevant EMDB volumes: EMD-22287
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Zinc transporters and their functional integration in mammalian cells.
Kambe et al. (2021)
4 more

4 mentions without citation

Metal transport mechanism of the cation diffusion facilitator (CDF) protein family - a structural perspective on human CDF (ZnT)-related diseases.
Barber-Zucker et al. (2021)
3 more