› 6xtx

Electron Microscopy
3.29Å resolution

CryoEM structure of human CMG bound to ATPgammaS and DNA

Released:
DOI: 10.2210/pdb6xtx/pdb
PDB entry 6xtx coloured by chain, front view.
PDB entry 6xtx coloured by chain, side view.
PDB entry 6xtx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes.
Rzechorzek NJ, Hardwick SW, Jatikusumo VA, Chirgadze DY, Pellegrini L
Nucleic Acids Res 48 6980-6995 (2020)
PMID: 32453425
Related structures: EMD-10619

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120979 (preferred)
Entry contents:
11 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (12 distinct):
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 904 amino acids
Theoretical weight: 102.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49736 (Residues: 1-904; Coverage: 100%)
Gene names: BM28, CCNL1, CDCL1, KIAA0030, MCM2
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 853 amino acids
Theoretical weight: 96.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25205 (Residues: 1-808; Coverage: 100%)
Gene name: MCM3
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 883 amino acids
Theoretical weight: 99.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P33991 (Residues: 1-863; Coverage: 100%)
Gene names: CDC21, MCM4
Sequence domains:
DNA replication licensing factor MCM5 Chain: 5
Molecule details ›
Chain: 5
Length: 734 amino acids
Theoretical weight: 82.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P33992 (Residues: 1-734; Coverage: 100%)
Gene names: CDC46, MCM5
Sequence domains:
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 821 amino acids
Theoretical weight: 93.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14566 (Residues: 1-821; Coverage: 100%)
Gene name: MCM6
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 719 amino acids
Theoretical weight: 81.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P33993 (Residues: 1-719; Coverage: 100%)
Gene names: CDC47, MCM2, MCM7
Sequence domains:
DNA replication complex GINS protein PSF1 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14691 (Residues: 1-196; Coverage: 100%)
Gene names: GINS1, KIAA0186, PSF1
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF2 Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 25.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y248 (Residues: 1-185; Coverage: 100%)
Gene names: CGI-122, DC5, GINS2, HSPC037, PSF2
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF3 Chain: C
Molecule details ›
Chain: C
Length: 216 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BRX5 (Residues: 1-216; Coverage: 100%)
Gene names: GINS3, PSF3
Sequence domains: GINS complex protein
DNA replication complex GINS protein SLD5 Chain: D
Molecule details ›
Chain: D
Length: 223 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BRT9 (Residues: 1-223; Coverage: 100%)
Gene names: GINS4, SLD5
Sequence domains:
Cell division control protein 45 homolog Chain: E
Molecule details ›
Chain: E
Length: 566 amino acids
Theoretical weight: 65.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75419 (Residues: 1-566; Coverage: 100%)
Gene names: CDC45, CDC45L, CDC45L2, UNQ374/PRO710
Sequence domains: CDC45-like protein
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 70 nucleotides
Theoretical weight: 21.39 KDa

Ligands and Environments

4 bound ligands:
Ligand ZN 5 x ZN
Ligand ADP 2 x ADP
Ligand AGS 3 x AGS
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.29Å
Relevant EMDB volumes: EMD-10619
Expression system: Homo sapiens

Quick links

Citations

5 review citations

DNA Polymerases at the Eukaryotic Replication Fork Thirty Years after: Connection to Cancer.
Pavlov et al. (2020)
4 more

22 mentions without citation

AAA+ ATPases: structural insertions under the magnifying glass.
Jessop et al. (2021)
21 more