› 6xty

Electron Microscopy
6.77Å resolution

CryoEM structure of human CMG bound to AND-1 (CMGA)

Released:
DOI: 10.2210/pdb6xty/pdb
PDB entry 6xty coloured by chain, front view.
PDB entry 6xty coloured by chain, side view.
PDB entry 6xty coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes.
Rzechorzek NJ, Hardwick SW, Jatikusumo VA, Chirgadze DY, Pellegrini L
Nucleic Acids Res 48 6980-6995 (2020)
PMID: 32453425
Related structures: EMD-10621

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130956 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 904 amino acids
Theoretical weight: 102.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49736 (Residues: 1-904; Coverage: 100%)
Gene names: BM28, CCNL1, CDCL1, KIAA0030, MCM2
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 853 amino acids
Theoretical weight: 96.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25205 (Residues: 1-808; Coverage: 100%)
Gene name: MCM3
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 863 amino acids
Theoretical weight: 96.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P33991 (Residues: 1-863; Coverage: 100%)
Gene names: CDC21, MCM4
Sequence domains:
DNA replication licensing factor MCM5 Chain: 5
Molecule details ›
Chain: 5
Length: 734 amino acids
Theoretical weight: 82.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P33992 (Residues: 1-734; Coverage: 100%)
Gene names: CDC46, MCM5
Sequence domains:
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 821 amino acids
Theoretical weight: 93.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14566 (Residues: 1-821; Coverage: 100%)
Gene name: MCM6
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 719 amino acids
Theoretical weight: 81.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P33993 (Residues: 1-719; Coverage: 100%)
Gene names: CDC47, MCM2, MCM7
Sequence domains:
DNA replication complex GINS protein PSF1 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14691 (Residues: 1-196; Coverage: 100%)
Gene names: GINS1, KIAA0186, PSF1
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF2 Chain: B
Molecule details ›
Chain: B
Length: 185 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y248 (Residues: 1-185; Coverage: 100%)
Gene names: CGI-122, DC5, GINS2, HSPC037, PSF2
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF3 Chain: C
Molecule details ›
Chain: C
Length: 216 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BRX5 (Residues: 1-216; Coverage: 100%)
Gene names: GINS3, PSF3
Sequence domains: GINS complex protein
DNA replication complex GINS protein SLD5 Chain: D
Molecule details ›
Chain: D
Length: 223 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BRT9 (Residues: 1-223; Coverage: 100%)
Gene names: GINS4, SLD5
Sequence domains:
Cell division control protein 45 homolog Chain: E
Molecule details ›
Chain: E
Length: 566 amino acids
Theoretical weight: 65.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75419 (Residues: 1-566; Coverage: 100%)
Gene names: CDC45, CDC45L, CDC45L2, UNQ374/PRO710
Sequence domains: CDC45-like protein
WD repeat and HMG-box DNA-binding protein 1 Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 1171 amino acids
Theoretical weight: 130.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75717 (Residues: 1-1129; Coverage: 100%)
Gene names: AND1, WDHD1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.77Å
Relevant EMDB volumes: EMD-10621
Expression system: Homo sapiens

Quick links

Citations

7 review citations

DNA Polymerases at the Eukaryotic Replication Fork Thirty Years after: Connection to Cancer.
Pavlov et al. (2020)
6 more

3 mentions without citation

CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes.
Rzechorzek et al. (2020)
2 more