› 6y1j

X-ray diffraction
1.13Å resolution

14-3-3 sigma in complex with IkappaBalpha pS63 peptide

Released:
DOI: 10.2210/pdb6y1j/pdb
PDB entry 6y1j coloured by chain, front view.
PDB entry 6y1j coloured by chain, side view.
PDB entry 6y1j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Interaction of an IκBα Peptide with 14-3-3.
Wolter M, Santo DL, Herman P, Ballone A, Centorrino F, Obsil T, Ottmann C
ACS Omega 5 5380-5388 (2020)
PMID: 32201828

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150627 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
NF-kappa-B inhibitor alpha Chain: P
Molecule details ›
Chain: P
Length: 14 amino acids
Theoretical weight: 1.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P25963 (Residues: 57-69; Coverage: 4%)
Gene names: IKBA, MAD3, NFKBI, NFKBIA

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
Ligand MG 1 x MG
Ligand GOL 7 x GOL
2 modified residues:
Ligand CSO 1 x CSO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: C2221
Unit cell:
a: 82.46Å b: 111.82Å c: 62.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.183
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Nipah virus W protein harnesses nuclear 14-3-3 to inhibit NF-κB-induced proinflammatory response.
Enchéry et al. (2021)

10 mentions without citation

Alleviation of Memory Deficit by Bergenin via the Regulation of Reelin and Nrf-2/NF-κB Pathway in Transgenic Mouse Model.
Shal et al. (2021)
9 more