6y5e

Electron Microscopy
3.15Å resolution

Structure of human cGAS (K394E) bound to the nucleosome (focused refinement of cGAS-NCP subcomplex)

Released:

Function and Biology Details

Reaction catalysed:
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-120988 (preferred)
Entry contents:
8 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (10 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 96 amino acids
Theoretical weight: 11.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q71DI3 (Residues: 39-134; Coverage: 71%)
Gene names: H3C13, H3C14, H3C15, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 9.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P62805 (Residues: 23-103; Coverage: 79%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 2-C Chain: C
Molecule details ›
Chain: C
Length: 107 amino acids
Theoretical weight: 11.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q16777 (Residues: 12-118; Coverage: 83%)
Gene names: H2AC20, H2AFQ, HIST2H2AC
Sequence domains:
Histone H2B type 1-K Chain: D
Molecule details ›
Chain: D
Length: 93 amino acids
Theoretical weight: 10.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O60814 (Residues: 33-125; Coverage: 74%)
Gene names: H2BC12, H2BFT, HIRIP1, HIST1H2BK
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chain: F
Molecule details ›
Chain: F
Length: 83 amino acids
Theoretical weight: 9.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P62805 (Residues: 21-103; Coverage: 81%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 2-C Chain: G
Molecule details ›
Chain: G
Length: 108 amino acids
Theoretical weight: 11.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q16777 (Residues: 12-119; Coverage: 84%)
Gene names: H2AC20, H2AFQ, HIST2H2AC
Sequence domains:
Histone H2B type 1-K Chain: H
Molecule details ›
Chain: H
Length: 94 amino acids
Theoretical weight: 10.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O60814 (Residues: 32-125; Coverage: 75%)
Gene names: H2BC12, H2BFT, HIRIP1, HIST1H2BK
Sequence domains: Core histone H2A/H2B/H3/H4
Cyclic GMP-AMP synthase Chain: K
Molecule details ›
Chain: K
Length: 362 amino acids
Theoretical weight: 42.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q8N884 (Residues: 161-522; Coverage: 69%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
DNA (153-MER) Chain: I
Molecule details ›
Chain: I
Length: 153 nucleotides
Theoretical weight: 47 KDa
DNA (153-MER) Chain: J
Molecule details ›
Chain: J
Length: 153 nucleotides
Theoretical weight: 47.46 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-10695
Expression system: Escherichia coli K-12