› 6ybi

X-ray diffraction
1.12Å resolution

RT structure of HEW Lysozyme obtained at 1.12 A resolution from crystal grown in a Mylar microchip.

Released:
DOI: 10.2210/pdb6ybi/pdb
PDB entry 6ybi coloured by chain, front view.
PDB entry 6ybi coloured by chain, side view.
PDB entry 6ybi coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips.
Gavira JA, Rodriguez-Ruiz I, Martinez-Rodriguez S, Basu S, Teychené S, McCarthy AA, Mueller-Dieckman C
Acta Crystallogr D Struct Biol 76 751-758 (2020)
PMID: 32744257

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 79.17Å b: 79.17Å c: 37.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.152

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Citations

4 citation in other articles

Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.
Gilbile et al. (2021)
3 more