6ybw

Electron Microscopy
3.1Å resolution

Structure of a human 48S translational initiation complex - 40S body

Released:
Source organism: Homo sapiens
Related structures: EMD-10775

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
PDBe Complex ID:
PDB-CPX-131135 (preferred)
Entry contents:
24 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (26 distinct):
Small ribosomal subunit protein eS4, X isoform Chain: C
Molecule details ›
Chain: C
Length: 263 amino acids
Theoretical weight: 29.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62701 (Residues: 1-263; Coverage: 100%)
Gene names: CCG2, RPS4, RPS4X, SCAR
Sequence domains:
Small ribosomal subunit protein uS17 Chain: B
Molecule details ›
Chain: B
Length: 157 amino acids
Theoretical weight: 18.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62280 (Residues: 1-157; Coverage: 99%)
Gene name: RPS11
Sequence domains:
Small ribosomal subunit protein uS12 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62266 (Residues: 1-143; Coverage: 100%)
Gene name: RPS23
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS4 Chain: D
Molecule details ›
Chain: D
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46781 (Residues: 1-194; Coverage: 100%)
Gene name: RPS9
Sequence domains:
Small ribosomal subunit protein eS7 Chain: G
Molecule details ›
Chain: G
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62081 (Residues: 1-194; Coverage: 100%)
Gene name: RPS7
Sequence domains: Ribosomal protein S7e
Small ribosomal subunit protein eS30 Chain: F
Molecule details ›
Chain: F
Length: 59 amino acids
Theoretical weight: 6.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62861 (Residues: 75-133; Coverage: 44%)
Gene name: FAU
Sequence domains: Ribosomal protein S30
Small ribosomal subunit protein eS27 Chain: H
Molecule details ›
Chain: H
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42677 (Residues: 1-84; Coverage: 100%)
Gene names: MPS1, RPS27
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS21 Chain: K
Molecule details ›
Chain: K
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63220 (Residues: 1-83; Coverage: 100%)
Gene name: RPS21
Sequence domains: Ribosomal protein S21e
Small ribosomal subunit protein uS8 Chain: J
Molecule details ›
Chain: J
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62244 (Residues: 1-130; Coverage: 100%)
Gene names: OK/SW-cl.82, RPS15A
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein eS17 Chain: M
Molecule details ›
Chain: M
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P08708 (Residues: 1-135; Coverage: 100%)
Gene names: RPS17, RPS17L
Sequence domains: Ribosomal S17
Small ribosomal subunit protein uS5 Chain: L
Molecule details ›
Chain: L
Length: 293 amino acids
Theoretical weight: 31.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P15880 (Residues: 1-293; Coverage: 100%)
Gene names: RPS2, RPS4
Sequence domains:
Small ribosomal subunit protein eS1 Chain: O
Molecule details ›
Chain: O
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61247 (Residues: 1-264; Coverage: 100%)
Gene names: FTE1, MFTL, RPS3A
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS2 Chain: N
Molecule details ›
Chain: N
Length: 295 amino acids
Theoretical weight: 32.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P08865 (Residues: 1-295; Coverage: 100%)
Gene names: LAMBR, LAMR1, RPSA
Sequence domains:
Small ribosomal subunit protein eS26 Chain: Q
Molecule details ›
Chain: Q
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62854 (Residues: 1-115; Coverage: 100%)
Gene name: RPS26
Sequence domains: Ribosomal protein S26e
Small ribosomal subunit protein uS11 Chain: P
Molecule details ›
Chain: P
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62263 (Residues: 1-151; Coverage: 100%)
Gene names: PRO2640, RPS14
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein eS6 Chain: S
Molecule details ›
Chain: S
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62753 (Residues: 1-249; Coverage: 100%)
Gene names: OK/SW-cl.2, RPS6
Sequence domains: Ribosomal protein S6e
Small ribosomal subunit protein eS8 Chain: R
Molecule details ›
Chain: R
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62241 (Residues: 1-208; Coverage: 100%)
Gene names: OK/SW-cl.83, RPS8
Sequence domains: Ribosomal protein S8e
Small ribosomal subunit protein eS24 Chain: T
Molecule details ›
Chain: T
Length: 133 amino acids
Theoretical weight: 15.46 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62847 (Residues: 1-133; Coverage: 100%)
Gene name: RPS24
Sequence domains: Ribosomal protein S24e
Eukaryotic translation initiation factor 1A, X-chromosomal Chain: q
Molecule details ›
Chain: q
Length: 144 amino acids
Theoretical weight: 16.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P47813 (Residues: 1-144; Coverage: 100%)
Gene names: EIF1A, EIF1AX, EIF4C
Sequence domains: Translation initiation factor 1A / IF-1
Eukaryotic translation initiation factor 1 Chain: p
Molecule details ›
Chain: p
Length: 113 amino acids
Theoretical weight: 12.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41567 (Residues: 1-113; Coverage: 100%)
Gene names: EIF1, SUI1
Sequence domains: Translation initiation factor SUI1
Small ribosomal subunit protein uS15 Chain: I
Molecule details ›
Chain: I
Length: 151 amino acids
Theoretical weight: 17.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62277 (Residues: 1-151; Coverage: 100%)
Gene name: RPS13
Sequence domains:
Eukaryotic translation initiation factor 3 subunit J Chain: z
Molecule details ›
Chain: z
Length: 258 amino acids
Theoretical weight: 29.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75822 (Residues: 1-258; Coverage: 100%)
Gene names: EIF3J, EIF3S1, PRO0391
Sequence domains: Translation initiation factor eIF3 subunit
Eukaryotic translation initiation factor 3 subunit C Chain: y
Molecule details ›
Chain: y
Length: 913 amino acids
Theoretical weight: 105.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99613 (Residues: 1-913; Coverage: 100%)
Gene names: EIF3C, EIF3S8
Sequence domains:
Small ribosomal subunit protein eS32 Chain: 9
Molecule details ›
Chain: 9
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62945 (Residues: 1-25; Coverage: 100%)
Gene name: RPL41
Sequence domains: Ribosomal protein L41
18S Ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1869 nucleotides
Theoretical weight: 603.13 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
Messenger RNA Chain: 7
Molecule details ›
Chain: 7
Length: 28 nucleotides
Theoretical weight: 9.17 KDa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-10775
Expression system: Escherichia coli