› 6ylu

X-ray diffraction
1.88Å resolution

14-3-3sigma in complex with BLNKpT152 phosphopeptide crystal structure

Released:
DOI: 10.2210/pdb6ylu/pdb
PDB entry 6ylu coloured by chain, front view.
PDB entry 6ylu coloured by chain, side view.
PDB entry 6ylu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
14-3-3sigma in complex with BLNKpT152 phosphopeptide crystal structure
Soini L, Leysen S, Davis J, Ottmann C
J. Struct. Biol. (2020)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152165 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
B-cell linker protein Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8WV28 (Residues: 147-158; Coverage: 3%)
Gene names: BASH, BLNK, SLP65

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 82.25Å b: 112.11Å c: 62.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.211
Expression systems:
  • Escherichia coli
  • Not provided

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