› 6yuo

X-ray diffraction
2.2Å resolution

Capsule O-acetyltransferase of Neisseria meningitidis serogroup A in complex with caged Gadolinium

Released:
DOI: 10.2210/pdb6yuo/pdb
PDB entry 6yuo coloured by chain, front view.
PDB entry 6yuo coloured by chain, side view.
PDB entry 6yuo coloured by chain, top view.
Source organism: Neisseria meningitidis serogroup A
Primary publication:
Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A.
Fiebig T, Cramer JT, Bethe A, Baruch P, Curth U, Führing JI, Buettner FFR, Vogel U, Schubert M, Fedorov R, Mühlenhoff M
Nat Commun 11 4723 (2020)
PMID: 32948778

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SacC Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 29.37 KDa
Source organism: Neisseria meningitidis serogroup A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68216 (Residues: 1-247; Coverage: 100%)
Gene name: sacC
Sequence domains: Uncharacterised protein family (UPF0227)

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand EDO 1 x EDO
Ligand GD3 2 x GD3
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41212
Unit cell:
a: 132.93Å b: 132.93Å c: 69.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Synthetic Glycans to Improve Current Glycoconjugate Vaccines and Fight Antimicrobial Resistance.
Del Bino et al. (2022)
2 more