› 6z3p

Electron Microscopy
2.8Å resolution

Structure of EV71 in complex with a protective antibody 38-3-11A Fab

Released:
DOI: 10.2210/pdb6z3p/pdb
PDB entry 6z3p coloured by chain, front view.
PDB entry 6z3p coloured by chain, side view.
PDB entry 6z3p coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analysis of protective antibodies targeting the threefold plateau of enterovirus 71.
Huang KA, Zhou D, Fry EE, Kotecha A, Huang PN, Yang SL, Tsao KC, Huang YC, Lin TY, Ren J, Stuart DI
Nat Commun 11 5253 (2020)
PMID: 33067459
Related structures: EMD-11061

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
PDBe Complex ID:
PDB-CPX-207424 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
VP1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.78 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: A0A2L1GIK5 (Residues: 566-862; Coverage: 14%)
Sequence domains: picornavirus capsid protein
VP2 Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.78 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: D4QGA2 (Residues: 70-323; Coverage: 30%)
Sequence domains: picornavirus capsid protein
VP3 Chain: C
Molecule details ›
Chain: C
Length: 242 amino acids
Theoretical weight: 26.54 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: D4QGA3 (Residues: 324-565; Coverage: 28%)
Sequence domains: picornavirus capsid protein
VP4 Chain: D
Molecule details ›
Chain: D
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: Q6YLE3 (Residues: 12-69; Coverage: 84%)
Sequence domains: Picornavirus coat protein (VP4)
heavy chain Chain: H
Molecule details ›
Chain: H
Length: 245 amino acids
Theoretical weight: 26.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
light chain Chain: L
Molecule details ›
Chain: L
Length: 233 amino acids
Theoretical weight: 25.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand SPH 1 x SPH
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-11061
Expression system: Homo sapiens

Quick links

Citations

4 citation in other articles

Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera.
Zhou et al. (2021)
3 more

2 mentions without citation

An ultrapotent pan-β-coronavirus lineage B (β-CoV-B) neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope.
Liu et al. (2022)
1 more