› 6zh6

Electron Microscopy
3.93Å resolution

Cryo-EM structure of DNA-PKcs:Ku80ct194

Released:
DOI: 10.2210/pdb6zh6/pdb
PDB entry 6zh6 coloured by chain, front view.
PDB entry 6zh6 coloured by chain, side view.
PDB entry 6zh6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dimers of DNA-PK create a stage for DNA double-strand break repair.
Chaplin AK, Hardwick SW, Liang S, Kefala Stavridi A, Hnizda A, Cooper LR, De Oliveira TM, Chirgadze DY, Blundell TL
Nat Struct Mol Biol 28 13-19 (2021)
PMID: 33077952
Related structures: EMD-11215

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146433 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-dependent protein kinase catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 4156 amino acids
Theoretical weight: 472.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P78527 (Residues: 1-4128; Coverage: 100%)
Gene names: HYRC, HYRC1, PRKDC
Sequence domains:
X-ray repair cross-complementing protein 5 Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13010 (Residues: 541-732; Coverage: 26%)
Gene names: G22P2, XRCC5
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.93Å
Relevant EMDB volumes: EMD-11215
Expression system: Escherichia coli

Quick links

Citations

15 review citations

X-ray scattering reveals disordered linkers and dynamic interfaces in complexes and mechanisms for DNA double-strand break repair impacting cell and cancer biology.
Hammel et al. (2021)
14 more