› 6zi8

X-ray diffraction
2.3Å resolution

X-ray diffraction structure of bovine insulin at 2.3 A resolution

Released:
DOI: 10.2210/pdb6zi8/pdb
PDB entry 6zi8 coloured by chain, front view.
PDB entry 6zi8 coloured by chain, side view.
PDB entry 6zi8 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Statistically correcting dynamical electron scattering improves the refinement of protein nanocrystals, including charge refinement of coordinated metals.
Blum TB, Housset D, Clabbers MTB, van Genderen E, Bacia-Verloop M, Zander U, McCarthy AA, Schoehn G, Ling WL, Abrahams JP
Acta Crystallogr D Struct Biol 77 75-85 (2021)
PMID: 33404527

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.4 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P01317 (Residues: 1-105; Coverage: 100%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: R3
Unit cell:
a: 81.28Å b: 81.28Å c: 33.31Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.162 0.238

Quick links

Citations

3 review citations

Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.
Chua et al. (2022)
2 more

1 mention without citation

Statistically correcting dynamical electron scattering improves the refinement of protein nanocrystals, including charge refinement of coordinated metals.
Blum et al. (2021)