› 6zjl

Electron Microscopy
4.3Å resolution

Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state

Released:
DOI: 10.2210/pdb6zjl/pdb
PDB entry 6zjl coloured by chain, front view.
PDB entry 6zjl coloured by chain, side view.
PDB entry 6zjl coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Key role of quinone in the mechanism of respiratory complex I.
Gutiérrez-Fernández J, Kaszuba K, Minhas GS, Baradaran R, Tambalo M, Gallagher DT, Sazanov LA
Nat Commun 11 4135 (2020)
PMID: 32811817
Related structures: EMD-11235

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176329 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
NADH-quinone oxidoreductase subunit 1 Chain: 1
Molecule details ›
Chain: 1
Length: 438 amino acids
Theoretical weight: 48.69 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56222 (Residues: 1-438; Coverage: 100%)
Gene names: TTHA0089, nqo1
Sequence domains:
NADH-quinone oxidoreductase subunit 2 Chain: 2
Molecule details ›
Chain: 2
Length: 181 amino acids
Theoretical weight: 20.31 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56221 (Residues: 1-181; Coverage: 100%)
Gene names: TTHA0088, nqo2
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit 3 Chain: 3
Molecule details ›
Chain: 3
Length: 783 amino acids
Theoretical weight: 86.66 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56223 (Residues: 1-783; Coverage: 100%)
Gene names: TTHA0090, nqo3
Sequence domains:
NADH-quinone oxidoreductase subunit 4 Chain: 4
Molecule details ›
Chain: 4
Length: 409 amino acids
Theoretical weight: 46.43 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56220 (Residues: 1-409; Coverage: 100%)
Gene names: TTHA0087, nqo4
Sequence domains: Respiratory-chain NADH dehydrogenase, 49 Kd subunit
NADH-quinone oxidoreductase subunit 5 Chain: 5
Molecule details ›
Chain: 5
Length: 207 amino acids
Theoretical weight: 23.89 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56219 (Residues: 1-207; Coverage: 100%)
Gene names: TTHA0086, nqo5
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NADH-quinone oxidoreductase subunit 6 Chain: 6
Molecule details ›
Chain: 6
Length: 181 amino acids
Theoretical weight: 20.26 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56218 (Residues: 1-181; Coverage: 100%)
Gene names: TTHA0085, nqo6
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NADH-quinone oxidoreductase subunit 9 Chain: 9
Molecule details ›
Chain: 9
Length: 182 amino acids
Theoretical weight: 20.11 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56224 (Residues: 1-182; Coverage: 100%)
Gene names: TTHA0092, nqo9
Sequence domains: 4Fe-4S binding domain
NADH-quinone oxidoreductase subunit 15 Chain: 7
Molecule details ›
Chain: 7
Length: 129 amino acids
Theoretical weight: 14.81 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SKZ7 (Residues: 1-129; Coverage: 100%)
Gene names: TTHA0496, nqo15
Sequence domains: NADH-quinone oxidoreductase chain 15
NADH-quinone oxidoreductase subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.15 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56217 (Residues: 1-119; Coverage: 100%)
Gene names: TTHA0084, nqo7
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-quinone oxidoreductase subunit 10 Chain: J
Molecule details ›
Chain: J
Length: 176 amino acids
Theoretical weight: 18.56 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56225 (Residues: 1-176; Coverage: 100%)
Gene names: TTHA0093, nqo10
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NADH-quinone oxidoreductase subunit 11 Chain: K
Molecule details ›
Chain: K
Length: 95 amino acids
Theoretical weight: 10 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56226 (Residues: 1-95; Coverage: 100%)
Gene names: TTHA0094, nqo11
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-quinone oxidoreductase subunit 12 Chain: L
Molecule details ›
Chain: L
Length: 606 amino acids
Theoretical weight: 65.19 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56227 (Residues: 1-606; Coverage: 100%)
Gene names: TTHA0095, nqo12
Sequence domains:
NADH-quinone oxidoreductase subunit 13 Chain: M
Molecule details ›
Chain: M
Length: 469 amino acids
Theoretical weight: 49.25 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56228 (Residues: 1-469; Coverage: 100%)
Gene names: TTHA0096, nqo13
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit 14 Chain: N
Molecule details ›
Chain: N
Length: 427 amino acids
Theoretical weight: 44.46 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56229 (Residues: 1-427; Coverage: 100%)
Gene names: TTHA0097, nqo14
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 365 amino acids
Theoretical weight: 41.03 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q60019 (Residues: 1-365; Coverage: 100%)
Gene names: TTHA0091, nqo8
Sequence domains: NADH dehydrogenase

Ligands and Environments

3 bound ligands:
Ligand SF4 7 x SF4
Ligand FMN 1 x FMN
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-11235

Quick links

Citations

8 review citations

Structure of respiratory complex I - An emerging blueprint for the mechanism.
Kampjut et al. (2022)
7 more