› 6zov

X-ray diffraction
2.19Å resolution

ENTEROPEPTIDASE IN COMPLEX WITH COMPOUND 6

Released:
DOI: 10.2210/pdb6zov/pdb
PDB entry 6zov coloured by chain, front view.
PDB entry 6zov coloured by chain, side view.
PDB entry 6zov coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting Enteropeptidase with Reversible Covalent Inhibitors To Achieve Metabolic Benefits.
Sun W, Zhang X, Cummings MD, Albarazanji K, Wu J, Wang M, Alexander R, Zhu B, Zhang Y, Leonard J, Lanter J, Lenhard J
J Pharmacol Exp Ther 375 510-521 (2020)
PMID: 33033171

Function and Biology Details

Reaction catalysed: 
Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161343 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enteropeptidase catalytic light chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P98073 (Residues: 785-1019; Coverage: 23%)
Gene names: ENTK, PRSS7, TMPRSS15
Sequence domains: Trypsin

Ligands and Environments

4 bound ligands:
Ligand GBS 4 x GBS
Ligand PGE 1 x PGE
Ligand EDO 6 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 52.925Å b: 147.517Å c: 147.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.25
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mechanism of Action of Small-Molecule Agents in Ongoing Clinical Trials for SARS-CoV-2: A Review.
Zhao et al. (2022)
8 other articles

2 mentions without citation

Inhibition of Hepatitis E Virus Replication by Novel Inhibitor Targeting Methyltransferase.
Hooda et al. (2022)
1 more