› 7c9w

Electron Microscopy
3.6Å resolution

E30 F-particle in complex with CD55

Released:
DOI: 10.2210/pdb7c9w/pdb
PDB entry 7c9w coloured by chain, front view.
PDB entry 7c9w coloured by chain, side view.
PDB entry 7c9w coloured by chain, top view.
Source organisms:
Primary publication:
Structures of Echovirus 30 in complex with its receptors inform a rational prediction for enterovirus receptor usage.
Wang K, Zhu L, Sun Y, Li M, Zhao X, Cui L, Zhang L, Gao GF, Zhai W, Zhu F, Rao Z, Wang X
Nat Commun 11 4421 (2020)
PMID: 32887891
Related structures: EMD-30319

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
PDBe Complex ID:
PDB-CPX-101286 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
VP1 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.09 KDa
Source organism: Echovirus E30
UniProt:
  • Canonical: A0A0F6T703 (Residues: 569-860; Coverage: 13%)
Sequence domains: picornavirus capsid protein
VP2 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 28.88 KDa
Source organism: Echovirus E30
UniProt:
  • Canonical: A0A0F6T703 (Residues: 70-330; Coverage: 12%)
Sequence domains: picornavirus capsid protein
VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.16 KDa
Source organism: Echovirus E30
UniProt:
  • Canonical: A8BJF8 (Residues: 331-568; Coverage: 11%)
Sequence domains: picornavirus capsid protein
VP4 Chain: D
Molecule details ›
Chain: D
Length: 68 amino acids
Theoretical weight: 7.44 KDa
Source organism: Echovirus E30
UniProt:
  • Canonical: Q8QWB2 (Residues: 2-69; Coverage: 99%)
Sequence domains: Picornavirus coat protein (VP4)
Complement decay-accelerating factor Chain: E
Molecule details ›
Chain: E
Length: 192 amino acids
Theoretical weight: 21.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08174 (Residues: 94-285; Coverage: 55%)
Gene names: CD55, CR, DAF
Sequence domains: Sushi repeat (SCR repeat)

Ligands and Environments

2 bound ligands:
Ligand SPH 1 x SPH
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-30319
Expression system: Homo sapiens

Quick links

Citations

2 review citations

Investigating the mechanism of Echovirus 30 cell invasion.
Liang et al. (2023)
1 more

2 mentions without citation

Structural basis for neutralization of an anicteric hepatitis associated echovirus by a potent neutralizing antibody.
Feng et al. (2021)
1 more