7cei

X-ray diffraction
2.3Å resolution

THE ENDONUCLEASE DOMAIN OF COLICIN E7 IN COMPLEX WITH ITS INHIBITOR IM7 PROTEIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-169934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.91 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 1-87; Coverage: 100%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chain: B
Molecule details ›
Chain: B
Length: 206 amino acids
Theoretical weight: 23.47 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: Q47112 (Residues: 371-576; Coverage: 36%)
Gene names: cea, colE7
Sequence domains:
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: I222
Unit cell:
a: 65.44Å b: 76.02Å c: 119.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.27
Expression system: Escherichia coli