› 7cy9

X-ray diffraction
1.36Å resolution

Crystal structure of a biodegradable plastic-degrading cutinase from Paraphoma sp. B47-9 solved by getting the phase from anomalous scattering of uncovalently coordinated arsenic (cacodylate).

Released:
DOI: 10.2210/pdb7cy9/pdb
PDB entry 7cy9 coloured by chain, front view.
PDB entry 7cy9 coloured by chain, side view.
PDB entry 7cy9 coloured by chain, top view.
Source organism: Paraphoma sp. B47-9
Entry authors: Suzuki K, Koitabashi M

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 19.95 KDa
Source organism: Paraphoma sp. B47-9
UniProt:
  • Canonical: A0A060N399 (Residues: 33-227; Coverage: 92%)
Sequence domains: Cutinase

Ligands and Environments

2 bound ligands:
Ligand CAD 3 x CAD
Ligand NA 4 x NA
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P1
Unit cell:
a: 34.886Å b: 43.517Å c: 51.724Å
α: 103.78° β: 92.42° γ: 101.49°
R-values:
R R work R free
0.124 0.122 0.166

Quick links